SEM to TEM / Pharos to Talos: Correlative Microscopy Guide

This tutorial provides a step-by-step guide on aligning images from a Pharos desktop SEM (Scanning Electron Microscope) to a Talos TEM (Transmission Electron Microscope) using MAPS software. The workflow covers reference point identification, coordinate system correlation, and mapping the same region of interest while accounting for differences in rotation. You can use the same alignment principles in this video in all Thermo Fisher SEM, FIB, and TEM systems with MAPS software. Key Topics Covered: • Alignment procedure for SEM to TEM correlative workflows • Reference point identification and coordinate transformation • Integration of SEM, FIB, and TEM data using MAPS software This tutorial demonstrates practical methods for achieving precise image correlation. Special thanks to Kristyna Bukvisova, Keriya Mam, Helen Oppong-Mensah, and Cigdem Ozsoy-Keskinbora for their contributions.