3D printed PDMS microfluidics tips and tricks (ESCARGOT)

Few years ago we published a method on how to make complex, 3D microfluidic devices in an easy way and how to incorporate external components in the microfluidic device. Years later I still get emails asking for more explanations about the method, and this video should help with some of the questions :) The method is called ESCARGOT (Embedded SCAffold RemovinG Open Technology) http://onlinelibrary.wiley.com/doi/10.1002/advs.201500125/abstract (open access) And original video: https://www.youtube.com/watch?v=7z8I7awRYY4 Looking for syringe pumps for your microfluidic device? We used a 3D printer for making them: https://www.youtube.com/watch?v=etUp4s1Bq-I In this video I explain some tips and tricks on how to make PDMS microfluidics devices using a standard 3D printer, ABS, and Acetone. Those devices can be used for lab on a chip, as analytical devices or practically for any other microfluidics research. Using this method is also possible to embed external components directly in the microfluidics device. For example LED, heating coils (for PCR), even whole arduino for color sensors. It is also possible to make a "ship-in-a-bottle" kind of devices. 00:00 Introduction 01:13 How to make the PDMS? 01:52 Why does my ABS does not dissolve? 02:52 How to make the channels? 06:30 How to make inlets and outlets? 08:55 How to make a flat design? 11:18 How long it takes to dissolve ABS? 13:21 Outro Canon In D Interstellar Mix by Kevin MacLeod Link: https://incompetech.filmmusic.io/song/6960-canon-in-d-interstellar-mix License: https://filmmusic.io/standard-license Holiday Weasel by Kevin MacLeod Link: https://incompetech.filmmusic.io/song/5517-holiday-weasel License: https://filmmusic.io/standard-license